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KMID : 0357419960260020173
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Korean journal of Virology
1996 Volume.26 No. 2 p.173 ~ p.180
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Cloning and Expression of Bovine Herpesvirus-1 gIII of Korean Isolate PQ Strain
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Abstract
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The gene encoding gIII of bovine herpesvirus type 1 (BHV-1) PQ strain was clone and expressed in baculovirus. Although the gIII gene is located in Hind III 1 fragment as the case of the other BHV-1 strains, differences in size and restriction
endonuclease site within the fragment were identified.
The gIII expression was predominantly detected on the surface on insect cells by indirect immunofluoresecnce assay using monoclonal antibody. The western blotting analysis also revealed the presence of expressed protein of a similar molecular
size
to
the original gIII protein.The immunogenicity of expressed protein were tested in guinea pigs. The immunized guinea pigs with expressed protein developed the neutralizing antibodies against BHV-1.
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